Publications

Eichler Laboratory – Selected Publications

Filamin a binds deleted in liver cancer 1 (DLC1) to promote its tumor suppressor activity and inhibit the SRF coactivator MRTF-A

Sergeev, M., Meier, M.A., Wohlleben, P., Rupprecht, L., Kupraszewicz-Hutzler, M., Hilgers, K., Hartner, A., Voegele, A.L., Atreya, R., Frey, Y., Srirangan, S., Eichler, J., Confais, C., Hédan, B., Jarry, U., Olayioye, M.A., Muehlich, S.

Neoplasia, 71, 101258, 2026.

DOI: https://doi.org/10.1016/j.neo.2025.101258.


Investigation of the Interactions and pH-Dependent Structural Changes in Helical Peptides by Molecular Dynamics Simulations

Zurmühl, S., Horn, A., Leukel, S., Eichler, J., Castiglione, K., Sticht, H.

J. Phys. Chem. B, 2026.

DOI: 10.1021/acs.jpcb.6c00802


Development and Characterization of a Human Papillomavirus-Based Nanoparticle Carrier for Heterologous Vaccine Antigens

Wang, Y., Bartelsen, N., Arnold, P., Müller-Schmucker, S. Weingärtner, C., Beutel, J. Eichler, J., Temchura, V., Damm, D. Überla, K.

Sci Rep 15, 32927, 2025.

DOI: https://doi.org/10.1038/s41598-025-19009-3


Versatile Features of an Antibody Mimetic Peptide and Its Variants

Dolles, S., Leukel, S., Gensberger-Reigl, S., Rohrhofer, A., Rauch-Wirth, L., Kaygisiz, K., Synatschke, C., Münch, J., Schmidt, B., Pischetsrieder, M., Eichler, J.

Journal of PeptideScience , 31:e70005 2025.

DOI: 10.1002/psc.70005


Oriented display of HIV-1 Env trimers by a novel coupling strategy enhances B cell activation and phagocytosis

Di Vincenzo, R., Beutel, J., Arnold, P., Wang, Y., Damm, D., Tannig, P., Lux, A., Temchura, V., Eichler, J. and Überla, K.

Front.Immunol. , 8(15), 1344346 2024.

DOI: 10.3389/fimmu.2024.1344346


Bind&Bite: Covalently stabilized heterodimeric coiled-coil peptides for the site-selective, cysteine-free chemical modification of proteins

Beutel, J., Tannig, P., Di Vincenzo, R., Schumacher, T., Überla, K. and Eichler, J.

RSC Chemical Biology , 4, 794 – 803 2023.

DOI: 10.1039/D3CB00122A


Computational Characterization of the Binding Properties of the HIV1-Neutralizing Antibody PG16 and Design of PG16-Derived CDRH3 Peptides

Deubler, M., Weißenborn, L., Leukel, S., Horn, A., Eichler, J., Sticht, H.

Biology , 12(6):824. 2023.

DOI: 10.3390/biology12060824


A Peptide Inhibitor of the Human Cytomegalovirus Core Nuclear Egress Complex

Alkhashrom S, Kicuntod J, Stillger K, Lützenburg T, Anzenhofer C, Neundorf I,  Marschall M, Eichler J.

Pharmaceuticals, 15(9):1040. 2022.

DOI: 10.3390/ph15091040


Smaller, Stronger, More Stable: Peptide Variants of a SARS-CoV-2 Neutralizing Miniprotein

Weißenborn L, Richel E, Hüseman H, Welzer J, Beck S, Schäfer S, Sticht H, Überla K, Eichler J

Int J Mol Sci,, 23(11):6309. 2022

DOI: 10.3390/ijms23116309


The crystal structure of the varicella-zoster Orf24-Orf27 nuclear egress complex spotlights multiple determinants of herpesvirus subfamily specificity

Schweininger, J., Kriegel, M., Häge, S., Conrad, M., Alkhashrom, S., Lösing, J., Weiler, S., Tillmanns, J., Egerer-Sieber, C., Decker, A., Lenac Roviš, T., Eichler, J., Sticht, H., Marschall, M., & Muller, YA.

Journal of Biological Chemistry, 101625. 2022.

DOI: 10.1016/j.jbc.2022.101625


A novel D-amino acid peptide with therapeutic potential (ISAD1) inhibits aggregation of neurotoxic disease-relevant mutant Tau and prevents Tau toxicity in vitro

Aillaud, I., Kaniyappan, S., Chandupatla, R. R., Ramirez, L. M., Alkhashrom, S., Eichler, J., Horn, A., Zweckstetter, M., Mandelkow, E., Sticht, H., & Funke, S. A.

Alzheimer’s research & therapy, 14(1), 15. 2022.

DOI: 10.1186/s13195-022-00959-z


A PROSS-designed extensively mutated estrogen receptor α variant displays enhanced thermal stability while retaining native allosteric regulation and structure

Kriegel M, Wiederanders HJ, Alkhashrom S, Eichler J, Muller YA.

Sci Rep. 2021.

DOI: 10.1038/s41598-021-89785-1


Exploring the Human Cytomegalovirus Core Nuclear Egress Complex as a Novel Antiviral Target: A New Type of Small Molecule Inhibitors

Alkhashrom, S., Kicuntod, J., Häge, S., Schweininger, J., Muller, Y. A., Lischka, P., Marschall, M., & Eichler, J.

Viruses, 2021.

DOI: 10.3390/v13030471


Properties of Oligomeric Interaction of the Cytomegalovirus Core Nuclear Egress Complex (NEC) and Its Sensitivity to an NEC Inhibitory Small Molecule

Kicuntod, J., Alkhashrom, S., Häge, S., Diewald, B., Müller, R., Hahn, F., Lischka, P., Sticht, H., Eichler, J., & Marschall, M.

Viruses, 2021.

DOI: 10.3390/v13030462


Exploring Viral Interference Using Peptides: Molecular Determinants of HIV-1 Inhibition by a Peptide Derived from Human Pegivirus-1 Envelope Protein E2

Hoffmann, R., Ruegamer, T., Schaubacher, J., Rohrhofer, A., Kirmess, P., Fiebig, K. M., Schmidt, B. and Eichler, J.

ChemMedChem 2020.

DOI: 10.1002/cmdc.202000892


Nuclear Egress Complexes of HCMV and Other Herpesviruses: Solving the Puzzle of Sequence Coevolution, Conserved Structures and Subfamily-Spanning Binding Properties

Marschall, M., Hage, S., Conrad, M., Alkhashrom, S., Kicuntod, J., Schweininger, J., Kriegel, M., Losing, J., Tillmanns, J., Neipel, F., Eichler, J., Muller, Y. A. and Sticht, H.

Viruses 2020.

DOI: 10.3390/v12060683


High-resolution crystal structures of two prototypical beta- and gamma-herpesviral nuclear egress complexes unravel the determinants of subfamily specificity

Muller, Y. A., Hage, S., Alkhashrom, S., Hollriegl, T., Weigert, S., Dolles, S., Hof, K., Walzer, S. A., Egerer-Sieber, C., Conrad, M., Holst, S., Losing, J., Sonntag, E., Sticht, H., Eichler, J. and Marschall,M.

J Biol Chem 2020.

DOI: 10.1074/jbc.RA119.011546


 Untargeted Proteomics-Based Profiling for the Identification of Novel Processing-Induced Protein Modifications in Milk

Meltretter, J., Wust, J., Dittrich, D., Lach, J., Ludwig, J., Eichler, J. and Pischetsrieder, M.

J Proteome Res 2020.

DOI: 10.1021/acs.jproteome.9b00630


Inhibition of HIV-1 infection by HPgV-1 derived peptides is affected by HPgV-1 genotype and HIV-1 coreceptor tropism

Ruegamer, T.; Hoffmann, R.; Rohrhofer, A.; Audebert, F., Salzberger, B.; Korn, K.; Schuster, P.; Eichler, J.; Schmidt, B.

Aids 2018

DOI: 10.1097/QAD.0000000000001926


The Hard Way towards an Antibody-Based HIV-1 Env Vaccine: Lessons from Other Viruses

Ringel, O.; Vieillard, V.; Debré, P.; Eichler, J.; Büning, H.; Dietrich, U.

Viruses 2018, 10, 197.

DOI: 10.3390/v10040197


Synthetic antibody mimics for the inhibition of protein – ligand interactions

Haußner, C.; Lach, J.; Eichler, J.

Curr.Opin.Chem.Biol. 2017, 40, 72-77.

DOI: 10.1016/j.cbpa.2017.07.001


The diversity and utility of amyloid fibrils formed by short amyloidogenic peptides

Al-Garawi, Z.S.; Morris, K.L.; Marshall, K.W.; Eichler, J.; Serpell, L.C.

Interface Focus 2017, 7:20170027.

DOI: 10.1098/rsfs.2017.0027


Peptide Paratope Mimics of the broadly neutralizing HIV-1 Antibody b12

Haußner, C.; Damm, D.; Nirschl, S.; Rohrhofer, A.; Schmidt, B.; Eichler, J.

ChemBioChem 2017, 18, 647-653.

DOI: 10.1002/cbic.201600621


Systematic analysis of phosphotyrosine antibodies recognizing single phosphorylated EPIYA-motifs in CagA of East Asian-type Helicobacter pylori strains

Lind, A.; Backert, S.; Hoffmann, R.; Eichler, J.; Yamaoka, Y.; Perez-Perez, G.I.; Torres, J.; Sticht, H.; Tegtmeyer, N.

BMC Microbiology 2016, 16:201.

DOI: 10.1186/s12866-016-0820-6


Synthetic Peptides as Protein Mimics

Groß, A.; Hashimoto, C.; Sticht, H.;Eichler, J.

Front.Bioeng.Biotechnol. 2016, 3:211.

DOI: 10.3389/fbioe.2015.00211


A monosaccharide residue is sufficient to maintain mouse and human IgG subclass activity and directs IgG effector functions to cellular Fc-receptors

Kao, D.; Danzer, H.; Collin, M.; Groß, A.; Eichler, J.; Stambuk, J.; Lauc, G.; Lux, A.; Nimmerjahn, J.

Cell Reports 2015, 13, 2376-2385.

DOI: 10.1016/j.celrep.2015.11.027


Virtual screening of a milk peptide database for the identification of food-derived antimicrobial peptides

Liu, Y.; Eichler, J.;Pischetsrieder, M.

Mol.Nutr.Food Res. 2015, 59, 2243-2254.

DOI: 10.1002/mnfr.201500182


Synthetic peptides mimicking the binding site of human acetylcholinesterase for its inhibitor fasciculin 2

Kafurke, U.; Erijman, A.; Aizner, Y.; Shifman, J.M.; Eichler, J.

J.Peptide Sci. 2015, 21, 723-730.

DOI: 10.1002/psc.2797


Turning peptide ligands into small-molecule inhibitors of protein-protein interactions

Hashimoto, C.; Eichler, J.

ChemBioChem 2015, 16, 1855-1856.

DOI: 10.1002/cbic.201500298


Ligand selectivity of a synthetic CXCR4 mimic

Groß, A.; Brox, R.; Damm, D.; Tschammer, N.; Schmidt, B.; Eichler, J.

Bioorg.Med.Chem. 2015, 23, 4050-4055.

DOI: 10.1016/j.bmc.2015.03.003


Enhancement and induction of HIV-1 infection through an assembled peptide derived from envelope glycoprotein gp120

Groß, A.; Rödel, K.; Kneidl, B.; Donhauser, N.; Mössl, M.; Lump, E.; Münch, J.; Schmidt, B.; Eichler, J.

ChemBioChem 2015, 16, 446-454.

DOI: 10.1002/cbic.201402545


Influence of Nonenzymatic Posttranslational Modifications on Constitution, Oligomerization and Receptor Binding of S100A12

Augner, K.; Eichler, J.; Utz, W.; Pischetsrieder, M.

PLoS One 2014.

DOI: 10.1371/journal.pone.0113418


Versatile trivalent C3-symmetric scaffolds and their use for covalent stabilization of the foldon trimer

Berthelmann, A.; Lach, J.; Gräwert M.A.; Groll, M.; Eichler

J. Org.Biomol.Chem. 2014, 12(16), 2606-2614.

DOI: 10.1039/C3OB42251H


Mimicking protein-protein interactions through peptide-peptide interactions: HIV-1 gp120 and CXCR4

Groß, A.; Möbius, K.; Haußner, C.; Donhauser, N.; Schmidt, B.; Eichler, J.

Frontiers in Immunology 2013.

DOI: 10.3389/fimmu.2013.00257


Identification of a Novel TGF-β-Binding Site in the Zona Pellucida C-terminal (ZP-C) Domain of TGF-β-Receptor-3 (TGFR-3)

Diestel, U.; Resch, M.; Meinhardt, K.; Weiler, S.; Hellmann, T.V.; Mueller, T.D.; Nickel, J.; Eichler, J.; Muller, Y.A.

PLoS One 2013, 8(6), e67214.

DOI: 10.1371/journal.pone.0067214


HIV-1 Fusion Is Blocked through Binding of GB Virus C E2D Peptides to the HIV-1 gp41 Disulfide Loop

Eissmann, K.; Mueller, S.; Sticht, H.; Jung, S.; Zou, P.; Jiang, S.; Groß, A.; Eichler, J.; Fleckenstein, B.; Reil, H.

PLOSone 2013, 8 (1), e54452.

DOI: 10.1371/journal.pone.0054452


Peptides presenting the binding site of human CD4 for the HIV-1 envelope glycoprotein gp120

Meier, J.; Kassler, K.; Sticht, H.; Eichler, J.

Beilstein J.Org.Chem.2012 8, 1858-1866, 2012.

DOI: 10.3762/bjoc.8.214


Exploring Converse Molecular Mechanisms of anti-HIV-1 Antibodies Using a Synthetic CXCR4 Mimic

Haußner, C.; Möbius, K.; Eichler, J.

Bioorg.Med.Chem.Lett. 2012, 22, 6099-6102.

DOI: 10.1016/j.bmcl.2012.08.035


The nuclear import of isoforms of the cytomegalovirus kinase pUL97 is mediated by differential activity of NLS1 and NLS2 both acting
through classical importin α binding

Webel, R.; Solbak, S.M.Ø.; Held, C.; Milbradt, C.; Groß, A.; Eichler, J.; Wittenberg, T.; Jardin, C.; Sticht, H.; Fossen, T.; Marschall, M.

J.Gen.Virol. 2012, 93, 1756-1768.

DOI: 10.1099/vir.0.040592-0


A Functionally Selective Synthetic Mimic of the HIV-1 Coreceptor CXCR4

Möbius, K.; Dürr, R.; Haußner, C.; Dietrich, U.; Eichler, J.

Chem.Eur.J. 2012, 18, 8292-8295.

DOI: 10.1002/chem.201200111


Structural basis for Species Selectivity in the HIV-1 gp120 – CD4 interaction: Restoring affinity to gp120 in murine CD4 mimetic peptides

Kassler, K.; Meier, J.; Eichler, J.; Sticht, H.

Adv.Bioinformatics 2011, Volume 2011, Article ID 736593.

DOI: 10.1155/2011/736593


Differential Downregulation of ACE2 by Spike Proteins of Severe Acute Respiratory Syndrome Coronavirus and Human Coronavirus NL63

Glowacka, I.; Bertram, S.; Herzog, P.; Pfefferle, S.; Steffen, I.; Muench, M.O.; Simmons, G.; Hofmann, H.; Kuri, T.; Weber, F.; Eichler, J.;
Drosten, C.; Pöhlmann, S.

J.Virol. 2010, 84, 1198-1205.

DOI: 10.1128/JVI.01248-09


Peptide ligands selected with CD4-induced epitopes on native dualtropic HIV-1 envelope proteinsmimic extracellular coreceptor domains and bind to HIV-1 gp120 independently of coreceptor usage

Dervillez, X.; Klaukien, V.; Dürr, R.; Koch, J.; Kreutz, A.; Haarmann, T.; Stoll, M.; Lee, D.; Carlomagno, T.; Schnierle, B.; Möbius, K.; Königs, C.;
Griesinger, C.; Dietrich, U.

J.Virol. 2010, 84, 10131-10138.

DOI: 10.1128/JVI.00165-10


HIV-derived peptide mimics

Möbius, K.; Eichler, J.

Drug Discovery Today: Technologies 2009, 6 (1-4), e19-e25.

DOI: 10.1016/j.ddtec.2009.09.001


The solution structure of pGolemi, a high affinity Mena EVH1 binding miniature protein, suggests explanations for
paralog-specific binding to Ena/VASP homology (EVH) 1 domains

Link, N.M.; Hunke, C.; Mueller, J.W.; Eichler, J.; Bayer, P.

Biol.Chem. 2009 390 (5-6),417-426.

DOI: 10.1515/BC.2009.045


Peptides as protein binding site mimetics

Eichler, J.

Curr.Opin.Chem.Biol. 2008 12, 707-713.

DOI: 10.1016/j.cbpa.2008.09.023


Synthetic Mimetics of the gp130 Binding Site for Viral Interleukin-6 as Inhibitors of the vIL-6 – gp130 Interaction

Sudarman, E.; Bollati-Fogolin, M.; Hafner, M.; Müller, W.; Scheller, J.; Rose-John, S.; Eichler, J.

Chem.Biol.Drug Des. 2008, 71, 494-500.

DOI: 10.1111/j.1747-0285.2008.00649.x


Structural/Functional Mimicry of the Binding Site of hYAP-WW Domain for Proline-Rich Ligands

Strijowski, U.; Hirsch, T.; Quintilla, A.; Wenzel, W.; Eichler, J.

Int.J.Peptide Res.Therap. 2007, 13(1-2),245-250.

DOI: 10.1007/s10989-006-9074-8


Synthetic Mimetics of the CD4 Binding Site of HIV-1 gp120 for the Design of Immunogens

Franke, R.; Hirsch, T.; Overwin, H.; Eichler, J.

Angew.Chem. 2007 119(8), 1275-1277 (Int.Ed. 46(8), 1253-1255).

DOI: 10.1002/anie.200603274


ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome

Taussig, M.J.; Stoevesandt, O.; Borrebaeck, C.A.K.; Bradbury, A.R.; Cahill, D.; Cambillau, C.; de Daruvar, A.; Dübel, S.; Eichler, J.; Frank, R.;
Gibson, T.J.; Gloriam, D.; Gold, T.; Herberg, F.W.; Hermjakob, H.; Hoheisel, J.D.; Joos, T.O.; Kallioniemi. O.; Koegll, M.; Konthur, Z.; Korn, B.;
Kremmer, E.; Krobitsch, S.; Landegren,U.; van der Maarel, S.; McCafferty, J.; Muyldermans, S.; Nygren, P.A.; Palcy, S.; Plückthun, A.; Polic, B.;
Przybylski, M.;Saviranta, P.; Sawyer, A.; Sherman, D.J.; Skerra, A.; Templin, M.; Ueffing, M.; Uhlén, M.

Nature Methods 2007 4, 13-17.

DOI:10.1038/nmeth0107-13


A Rationally Designed Synthetic Mimic of the Discontinuous CD4 Binding Site of HIV-1 gp120

Franke, R.; Hirsch, T.; Eichler, J.

J.Recept.Sig.Transd. 2006, 26, 453-460.

DOI: 10.1080/10799890600923179


Abrogation of vIL-6 induced signaling by intracellular retention and neutralization of vIL-6 with an anti vIL-6 single-chain antibody selected by phage display

Kovaleva, M.; Bußmeyer, I.; Rabe1, B.; Sudarman, E.; Eichler, J.; Conrad, U.; Rose-John, S.; Scheller, J.

J.Virolol. 2006, 80, 8510-8520.

DOI:10.1128/JVI.00420-06


Structure-Based Synthetic Mimicry of Discontinuous Protein Binding Sites: Inhibitors of the Interaction of Mena EVH1 Domain with Proline-Rich Ligands

Hunke, C.; Hirsch, T.; Eichler, J.

ChemBioChem 2006, 7(8), 1258-1264.

DOI: 10.1002/cbic.200500465


Forced dimerization of gp130 leads to constitutive STAT3 activation, cytokine independent growth and blockade of differentiation of embryonic stem cells

Stuhlmann-Laisz, C.; Lang, S.; Chalaris, A.;  Sudarman, E.; Eichler, J.; Klingmüller, U.; Samuel, M.;  Ernst, M.; Rose-John, S.; Scheller, J.

Molecular Biology of the Cell 2006, 17, 2986-2995.

DOI: 10.1091/mbc.E05-12-1129


Peptide ligation through click chemistry for the generation of assembled and scaffolded peptides

Franke, R.; Doll, C.; Eichler, J.

Tetrahedron Lett. 2005, 46(26), 4479-4482.

DOI:10.1016/j.tetlet.2005.04.107


Synthetic Peptide Arrays and Peptide Combinatorial Libraries for the Exploration of Protein-Protein Interactions and the Design of Protein Inhibitors

Eichler, J.

Comb.Chem.High Throughput Screen. 2005,8(2), 135-143.

DOI: 10.2174/1386207053258497


Post-Genomic Chemistry

Varfolomeyev, S.; Efremenko, E.; Beletskaya, I.; Bertini, I.; Blackburn, G.M.; Bogdanov, A.; Cunin, R.; Eichler, J.; Galaev, I.; Gladyshev, V.;
O’Hagan, D.; Haertle, T.; Jarv, J.; Karyakin, A.; Kurochkin, I.; Mikolajczyk, M.; Poroikov, V.; Sakharov, I.; Spener, F.; Voyer, N.; Wild, J.

Pure Appl.Chem. 2005, 77(9): 1641-1654.

DOI: 10.1351/pac200577091641


Loops on Loops: Generation of Complex Scaffolded Peptides Presenting Multiple Cyclic Fragments

Franke, R.; Doll, C.; Wray V.; Eichler, J.

Org.Biomol.Chem. 2004, 2(19): 2847-2851.

DOI: 10.1039/b403720k


Development of a monoclonal antibody-based immunoabsorbent assay for the binding of gp130 to the IL-6/IL-6R complex and its competitive inhibition

Scheller, J.; Kovaleva, M.; Rabe, B.; Eichler, J.; Kallen, K.J.; Rose-John, S.

J.Immunol.Methods 2004, 291(1-2): 93-100, 2004.

DOI: 10.1016/j.jim.2004.05.002


Rational and Random Strategies for the Mimicry of Discontinuous Protein Binding Sites

Eichler, J.

Protein Peptide Lett. 2004, 11(4): 281-290, 2004.

DOI: 10.2174/0929866043406931